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ELISpot

Questions and comments on ELISpot.

118 topics in this forum

  1. Human pan IgG ELISpot - dark membrane and white spots

    By Guest Emily,
    • 2 replies
    • 66 views

  2. Validation of ELISpot

    By Guest Margot,
    • 0 replies
    • 40 views

  3. Effector target ratio for low antigen load

    By Gael,
    • 1 reply
    • 37 views

  4. Peptide length for ELISPOT?

    By Guest Guest Andrew,
    • 4 replies
    • 181 views

  5. IFN-gamma Elispot with human PBMC activated by adherent tumor cells

    By Guest Esen Aral,
    • 1 reply
    • 62 views

  6. Question about viability of frozen splenocytes after thawing (ELISpot)

    By Guest A Le Heron,
    • 1 reply
    • 87 views

  7. Odd spots in negative control wells

    By Guest Tim,
    • 1 reply
    • 49 views

  8. Help with antigen-specific ELISpot assay

    By Guest Federico Ruiz Moreno,
    • 6 replies
    • 288 views

  9. How long can a coated plate last in the fridge?

    By Guest Emily,
    • 2 replies
    • 179 views

  10. Question about RPMI and cell volume

    By Guest Federico Ruiz Moreno,
    • 1 reply
    • 138 views

  11. Issues with Elispot

    By Saswati Emma Mohr lab,
    • 1 reply
    • 247 views

  12. Buffer solution used for making coating anti human IgG for B cells ELISpot

    By Guest Syabila,
    • 3 replies
    • 354 views

  13. TMB for HRP SAME FOR ELISA AND ELISPOT

    By Guest Camila Enriquez,
    • 1 reply
    • 525 views

  14. Problems with central membrane white spot and retained blue color

    By Guest Saswati Bhattacharya,
    • 1 reply
    • 219 views

  15. Plate Preparation protocol of using Pre-coated ELISpot clear plate

    By Guest Syabila,
    • 5 replies
    • 250 views

  16. Blurry spots in antigen-specific wells

    By Guest Mason,
    • 2 replies
    • 257 views

  17. No clear spots in mouse IFN-γ ELISpot

    By Guest Rain,
    • 5 replies
    • 331 views

  18. IFN-g ELISpot using TMB

    By Guest Hayan,
    • 1 reply
    • 188 views

  19. MICE ELISpot Assay

    By Guest Ivan Ssali,
    • 1 reply
    • 204 views

  20. Shelf life of no pre-coated plates.

    By Guest Mathilde,
    • 2 replies
    • 271 views

  21. TMB used in B cell ELISpot development (3825-2H)

    By Guest Mason,
    • 5 replies
    • 420 views

  22. change of background color after development(B cell ELISpot)

    By Guest Eunju Jung,
    • 1 reply
    • 248 views

  23. Finding Lot Numbers for kits

    By Guest Stephen,
    • 2 replies
    • 154 views

  24. IFN gamma ELISpot on splenocytes form humanized mice_340-2HST-02 kit

    By FLAVIA,
    • 3 replies
    • 271 views

  25. Purple center area

    By Jun,
    • 1 reply
    • 257 views
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