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Showing content with the highest reputation on 08/27/2021 in all areas

  1. Dear Idania, I would say in general that you results look very good overall. There would be no difficulty counting this plate accuratly on IRIS or ASTOR. But I see you are using another reader where a tone difference in membrane-color throws off the spot-counting algorithm. I understand the problem. Well D4-D9 are confluent with spots and the substrate often leave a slight purple tone to them. However, the membrane color difference between well D1 and D2 is striking. I think the use of tween can contribute to the problem you are seeing. I therefore would suggest another protoc
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