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akira

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  1. Antigen-specific B cell ELISPOT

    Dear Christian, Thank you for your kind support!! Let me contact if I need further supports. Sincerely, Akira
  2. Antigen-specific B cell ELISPOT

    Dear Jahnmatz, Thank you for quick response!! I will follow the recommendation, and please let me contact you again if I need further support. Sincerely,
  3. Antigen-specific B cell ELISPOT

    Dear Staffs, This is my first time to perform B cell ELISPOT and plan to detect antigen (drug)-specific IgG or IgM response. My plan is to coat the plate (MultiScreenHTS IP Filter Plate, Millipore) with specific antigen, and use anti-human IgG mAbs MT78/145, biotinylated (3850-6-250) or anti-human IgM mAb MT22, biotinylated (3880-6-250), followed by adding Streptavidin-ALP for ELISpot (3310-10-1000). I am able to detect antigen-specific B cells in PBMC with the similar frequency with flu-specific B cells by FACS, although it is very low frequency. 1. I appreciate if you provide the recommended dilution rate of these Abs to begin with. 2. I wonder if I should use antigen-specific B cell (sorted by FACS) or whole B cells in this ELISPOT. I appreciate if you give the advice for it.. Sincerely,
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