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Hugo

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  1. ELISpot cell dulitions

    Hi all, I understand you often have to plate multiple cell dilutions in the ELISpot to determine the appropriate number of cells that will produce good quality spots. My question is, if all your cell dilutions produce spots, how do you go about deciding which spot counts to use? Do you average them all? Which cell dilution do you "believe"? Thanks!
  2. ELISpot system suitability

    Hi all, what would be some parameters one could use for system suitability of an ELISpot and/or Fluorospot? Thanks!
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