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Keisuke

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  1. Dear Christian, That will be great. I will try that. Thank you very much. Keisuke
  2. Dear Christian, Thank you for your kind advice. Dose "reversed B-cell ELISPOT protocol" mean FluoroSpot? Is it possible with biotinylated antigen and ELISPOT reader? Even in the reversed protocol, I suspect that the “sticky” IgG trapped on the membrane also will bind to the biotinylated antigens used for detection. We also thought about the possibility of the occult infection or past infection because the donors are elder people. However, such phenomenon is not rare and we currently think it is unlikely. On the other hand, if the spot is representing truly specific IgG, it i
  3. Dear Mabtech staff and researchers, I am conducting HBV Surface antigen (HBsAg)-specific B cell IgG ELISPOT assay using U-Cytech kit (CT780-PR5). Attached is the image of the result. This time PBMCs from some healthy vaccinated (i.e. positive for anti-HBs) donors and clinically uninfected donors with no history of vaccination (i.e. negative for serum HBsAg, anti-HBsAg and anti-HBcAg) were stimulated with rIL2 and R848 for five days. PVDF membrane plate (Millipore, MSIPS4510) was coated with four recombinant SHBs antigens, PBS or IgG capture antibodies. The problem is that some
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