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  1. Hello Luc, We have done tests to see how the incubation time affects the coating efficiency. The tests were made at room temp and the amount of mAb bound to the membrane was determined by measuring the free mAb in the PBS (the buffer used for coating) at different times. In the graph shown we added 1.5 ug of mAb in 100 ul and after 8 h close to 100% of the added mAb was bound. Shortening the coating time to less than 8 h yielded less efficient coating and a 4 h incubation resulted in approximately 85% bound mAb. Please note that this was done with EtOH treated PVDF plates; the use of non-trea
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