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julia@mabtech.com

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About julia@mabtech.com

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    Julia Salazar Gustavsson

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  1. Red blood cells in ELISpot

    Hi! Generally a limited amount of erythrocytes is likely to have little or no effect on ELISpot results. However, as the assay is dependent on the cytokine-secreting cells being in relatively close contact with the antibody coated membrane and erythrocytes may compete for space, there is a limit to how much erythrocytes you can have before they start to interfere. So, even if it - in most cases - would work just fine, the risk is that you will have varying amounts of “contaminating” erythrocytes in different cell preparations and therefore it may be safest to still try to remove them. Apart from lysing the erythrocytes, which most seem to do even though it’s been reported to give varying results, people use Ficoll centrifugation which is probably a better way of doing it. Kind regards, Julia
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