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Caroline

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  1. Caroline

    Dual ELISpot

    Dear Christian, Thank you very much for your answer. Unfortunately we don't have the new mabtech reader available on site. I started my first comparison experiment last week and it's very difficult for a beginner to decide which gain etc to put on the machine. I tried to compare my ELISPOT and fluorospot counts (IFNg Elispot and IFNg/IL-17 Fluorospot) and there is a much high number of spots with the Fluorospot. I tried to correct them manually but there still very high and it is honestly quite difficult when it is super bright to make a difference between 2,3,5 spots. I have a
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