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Hi Christian, We are currently experiencing high background with the IgM detection within our IgA/G/M fluorospot. This issue seems to present wherever cells have been plated IgA and IgG detection works well ! We have followed the Mabtech IgA/G/M fluorospot kit protocol exactly Any ideas on how the background (non specific binding) for IgM-cy5 can be reduced ? Attached are our plate fluorospot read outs and plate plan Thanks Fluorospot issue Cy5.pptx