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Guest Marine

IL-2 specific activity

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Guest Marine

Hello,

Would you know the specific activity in IU/ug of the human IL-2 provided in your Fluorospot kit (IgG, IgA, IgM fluorospot)?

Thanks

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Hi!

The IL-2 supplied in the ELISpot kit is used for stimulation of memory B cells in combination with R848. The activity of the IL-2 is confirmed to give more than 500 spots per 50 000 PBMC (per well) with pre-stimulation using 10 ng of IL-2/ml and 1 ug/ml of R848. The biological activity of IL-2 has not been compared with an international reference reagent to establish the number of units.

Best regards!

Peter

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