Guest Sara Posted January 27, 2015 Report Share Posted January 27, 2015 Hi, I am interested in transitioning from ELISPOT to FluoroSpot. I currently analyze T-cell activation in antigen-stimulated PBMCs with ELISPOT for IFNg and IL17A. It would be nice to change to FluoroSpot, and get the information on double secreting cells, but I am worried that there may be a difference in the result of cells producing single cytokines. Do you have any experience with this? I am also interested in the triple FluoroSpot for IFNg/IL17A/IL22, if it is compareable to my ELISPOT assays. Thanks! Link to comment Share on other sites More sharing options...
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