Guest Lucia Posted January 28, 2015 Report Share Posted January 28, 2015 Hi, We are now doing FluoroSpot with your IFN-g/IL-2 kit, when we read the protocol, we have such questions ,could you please help to give some comments: The step of dilution of detection antibody, it was written with PBS-0.1%BSA. Can we use PBS- serum instead? What is the suggested concentration of serum?And for the step of filtering the antibodies, must the filter be sterile one? Is non-sterile one ok? Why we need to filter the antibodies? I want to know what is filtered by this step? Thanks! Link to comment Share on other sites More sharing options...
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