apo-ELISAs and cerebrospinal fluid

[Guest Olimpia Gamucci]

Hello,

 

we would like to know if the Human apoE ELISA PRO kit (3712-1HP-2) can be used on Cerebrospinal fluid (CSF), human, and if you have an idea about the dilution of such sample.

 

Thank you.

 

Olimpia Gamucci

[Guest Sten Braesch-Andersen]

Dear Olimpia,

The authors of the paper below used the Mabtech apoE ELISA for spinal fluid. They obtained values around 5 ug/ml. Most likely a dilution of 1000X would be apropriate, but you may have to try two or three different dilutions in your first experiment.

 

Best wishes,

Sten Braesch-Andersen

 

Translational spatial task and its relationship to HIV-associated neurocognitive disorders and apolipoprotein E in HIV-seropositive women.

Morales D, Acevedo SF, Skolasky RL, Hechavarria R, Santiago S, De La Torre T, Maldonado E, Wojna V.

J Neurovirol. 2012 Dec;18(6):488-502. doi: 10.1007/s13365-012-0128-8. Epub 2012 Sep 13.

 

[Jens@mabtech.com]

Hi Olimpia and Sten,

 

Here's yet another paper using Mabtech's ELISA-kit to measure apoE in cerebrospinal fluid:

 

https://www.ncbi.nlm.nih.gov/pubmed/24277910

 

Kind regards,

Jens

[Lena@mabtech.com]

Hi!

I just want  to add two interesting articles to this discussion:

 

Van Harten et al. are also using Mabtech's apoE ELISA to analyze apoE in CSF and plasma, they dilute the samples 1:2000 and 1:20000, respectively.

 

 Van Harten et al. (2017) CSF ApoE predicts clinical progression in nondemented APOEε4 carriers. Neurobiol Aging.

 

Several apolipoproteins are present in cerebrospinal fluid, Slot et al. are using Mabtech's apoA1 ELISA du analyze apoA1 in CSF and plasma, and for apoA1 they dilute CSF and plasma 1:1000 and 1:100000, respectively.

 

Slot et al. (2017) Apolipoprotein A1 in Cerebrospinal Fluid and Plasma and Progression to Alzheimer's Disease in Non-Demented Elderly. J Alzheimers Dis.

 

Best regards,

Lena