Guest Marine Posted September 16, 2020 Report Share Posted September 16, 2020 Hello, Would you know the specific activity in IU/ug of the human IL-2 provided in your Fluorospot kit (IgG, IgA, IgM fluorospot)? Thanks Quote Link to comment Share on other sites More sharing options...
Jahnmatz Posted September 16, 2020 Report Share Posted September 16, 2020 Hi! The IL-2 supplied in the ELISpot kit is used for stimulation of memory B cells in combination with R848. The activity of the IL-2 is confirmed to give more than 500 spots per 50 000 PBMC (per well) with pre-stimulation using 10 ng of IL-2/ml and 1 ug/ml of R848. The biological activity of IL-2 has not been compared with an international reference reagent to establish the number of units. Best regards! Peter Quote Link to comment Share on other sites More sharing options...
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