Guest Eunice Kiamba Posted October 22, 2020 Report Share Posted October 22, 2020 Just running a dual fluorospot IgG/IgM and can confirm spots using ELISpot but when I run Fluorospot for the same, I only have responses in the total IgG/IgM wells Any help please Quote Link to comment Share on other sites More sharing options...
Guest Eunice Kiamba Posted October 22, 2020 Report Share Posted October 22, 2020 I am Using the AID ELISpot reading machine Quote Link to comment Share on other sites More sharing options...
Jahnmatz Posted October 22, 2020 Report Share Posted October 22, 2020 Hi Eunice I will gladly help you with you. However, I think that I need some more information about you assay first. As I understand it you are seeing antigen-specific IgG and IgM spots in ELISpot, but not in FluoroSpot when you run a dual IgG/IgM assay? Is that right? Since you see IgG and IgM spots in the total wells, there seems to be no problem with the detection antibodies or the cells. Please provide some more info on the protocols (you dont have to share specifics, only the general protocol that you use). Best regards Peter Quote Link to comment Share on other sites More sharing options...
Christian@mabtech.com Posted October 22, 2020 Report Share Posted October 22, 2020 Agree with Peter. But please also add some well images from the AID reader. Add them into a powerpoint and write in what is what. Then upload here. Will help a lot. Quote Link to comment Share on other sites More sharing options...
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