Jump to content

Fluorospot IL-4 positive control

Guest Leonie

Recommended Posts


I would like to perform the IFNg/IL-4 Fluorospot on PBMCs stimulated with a vaccine antigen in order to see if the vaccine induces predominantly a Th1 or Th2 response. Do you have a recommenation for a positive control for IL-4 secretion? E.g. PBMCs of a patient with a specific helminth infection, malaria,...? 



Link to comment
Share on other sites

Hello Leonie,

For human IFNg/IL4 I would recommend using either (AntiCD3+antiCD28) or PHA. For IL-4 secretion it is good to go up 100K-150K cells/well in order to get good response. 

Combining antiCD3 and antiCD28 will give the response a push. Works also without but spots do become stronger and often more plentiful. 

For PHA I would recommend using the same cell numer. 


Link to comment
Share on other sites

Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.


  • Create New...