Guest Ali Posted January 20, 2022 Report Share Posted January 20, 2022 Hi, I was coating 2 FluoroSpot plates at time. Due to some fault in the pipette it took 2 minutes (recommended time in 1 minute maximum) to start washing after adding ethanol. Will this be a problem in the assay? Many thanks and best regards! Quote Link to comment Share on other sites More sharing options...
Christian@mabtech.com Posted January 20, 2022 Report Share Posted January 20, 2022 Hi Ali, In the last decade or so Millipore produces FluoroSpot plates where the underdrain has a very resilient design which makes them much less prone to leakage than was the norm maybe 15 years ago. EtOH activation for 2min should not be a problem. The fear is that the excessive etoh activation will cause leakage whereby cell cultures and reagents gets sucked down below the membrane and get trapped within the underdrain attached to the bottom of the plate. This leads to very strange results and a ruined assay. But luckily these problems are a thing of the past. Jens@mabtech.com 1 Quote Link to comment Share on other sites More sharing options...
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