Jump to content

Incubated FlouroSpot plates with ethanol more than 1 minute

Guest Ali

Recommended Posts


I was coating 2 FluoroSpot plates at time. Due to some fault in the pipette it took 2 minutes (recommended time in 1 minute maximum) to start washing after adding ethanol. Will this be a problem in the assay?

Many thanks and best regards!   

Link to comment
Share on other sites

Hi Ali,

In the last decade or so Millipore produces FluoroSpot plates where the underdrain has a very resilient design which makes them much less prone to leakage than was the norm maybe 15 years ago. EtOH activation for 2min should not be a problem. 

The fear is that the excessive etoh activation will cause leakage whereby cell cultures and reagents gets sucked down below the membrane and get trapped within the underdrain attached to the bottom of the plate. This leads to very strange results and a ruined assay. 

But luckily these problems are a thing of the past.



Link to comment
Share on other sites

Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.


  • Create New...