Guest Sara Posted January 27, 2015 Report Share Posted January 27, 2015 Hi, I have recently started analyzing T cell activation with GM-CSF ELISPOT. PBMCs are stimulated with various antigens and my read-out is GM-CSF as well as IL-2, IFN-g and IL-17A. I am concerned that I may get false positives, especially in the GM-CSF assay, due to LPS-contamination of my antigens (I get a lot of spots) and that the signal may originate from monocytes and not T cells. Do you have any experience of this and/or any advice on how to avoid this problem. Thanks! Link to comment Share on other sites More sharing options...
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