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ELISpot

Questions and comments on ELISpot.

82 topics in this forum

  1. shelf life of pre-coated plates

    By Guest Stanislava,
    • 1 reply
    • 0 views

  2. Use of FCS for blocking

    By Guest thomson,
    • 3 replies
    • 3 views

  3. Spots in no stimuli wells when using mouse IFNg splenocytes

    By Guest Subeena Sood,
    • 4 replies
    • 173 views

  4. too many negative spot of IFNg ELISpot

    By mKim,
    • 2 replies
    • 78 views

  5. Colors of IFNg spot

    By Guest Tan Trieu Nguyen,
    • 1 reply
    • 89 views

  6. Using APCs in ELISpot with CD8+ T cells

    By Guest Nils,
    • 1 reply
    • 95 views

  7. Purple wells when developing Mouse IFN-y ELISPOT plus kit 3321-4AST-2

    By Guest Rongji,
    • 1 reply
    • 155 views

  8. IFNg ELISPOT: Inhibition of background spots in wells for which the donor is seronegative

    By Guest José Manuel Marín,
    • 1 reply
    • 154 views

  9. Poorly developed ELISpot membrane

    By Guest Lena,
    • 3 replies
    • 254 views

  10. Purple spot background

    By Guest Idania,
    • 1 reply
    • 183 views

  11. High Background in IFNg ELISpot for mouse spleenocytes

    By Guest Sajib,
    • 5 replies
    • 182 views

  12. Background Problem with Mouse IFN-gamma ELISPOT assay

    By Guest Pavel,
    • 9 replies
    • 2.3k views

  13. Antigen-specific IgG spots by antibody-negative donors

    By Keisuke,   
    • 4 replies
    • 162 views

  14. Stimuli for ELISPOT

    By Guest Alok Tembhurne,
    • 11 replies
    • 1.1k views

  15. IFN-Gamma ELISPOT No Development?

    By Guest Eva,
    • 6 replies
    • 856 views

  16. HBsAg-specific B cell ELISPOT assay

    By Guest E.J. Jung,
    • 8 replies
    • 321 views

  17. Dark "clumps" in peptide-stimulated samples

    By Guest Tetiana,
    • 1 reply
    • 222 views

  18. Total mouse IgG ELISPOT - not many spots?

    By Guest Lucy,
    • 9 replies
    • 253 views

  19. How long stable is the SFC on plate membrane after air-dry

    By mKim,
    • 2 replies
    • 169 views

  20. IQ/OQ of Eli Scan Plus reader

    By mKim,
    • 2 replies
    • 402 views

  21. Blank areas in IFNg ELISpot with human PBMCs

    By Guest Lena,
    • 4 replies
    • 260 views

  22. Scientist

    By Guest Wendy,
    • 2 replies
    • 192 views

  23. No spots for IFN-gamma

    By Guest Kenneth,
    • 5 replies
    • 339 views

  24. Antigen-specific B cell ELISPOT

    By akira,
    • 12 replies
    • 455 views

  25. ELISPOT B cell IgG background and no spot

    By Guest Calméjane,
    • 3 replies
    • 245 views
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