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ELISpot

Questions and comments on ELISpot.

94 topics in this forum

  1. Purple center area

    By Jun,
    • 1 reply
    • 33 views

  2. how to add stimulants and how many micrograms of each protein is appropriate

    By Guest TANG,
    • 1 reply
    • 46 views

  3. how to add stimulants and how many micrograms of each protein is appropriate

    By Guest TANG,
    • 1 reply
    • 45 views

  4. Bovine ASC elispot

    By Guest Analía,
    • 8 replies
    • 79 views

  5. Question about ASC ELISPOT or FLuorospot for sheep

    By Analia,
    • 2 replies
    • 20 views

  6. Question about the standard curve of ELISA Flex

    By Guest Liu,
    • 3 replies
    • 52 views

  7. About precipitate, membrane staining and negative controls in ELISpot assay

    By Yaice Sandoval,
    • 5 replies
    • 178 views

  8. High background level and Repeatability

    By Xian Yang,
    • 3 replies
    • 154 views

  9. 'Cells alone'/'no stimuli' wells with high number of spots

    By Guest Carlos,
    • 4 replies
    • 198 views

  10. Rabbit IFN-r ELISPOT-Abnormal dark background

    By Guest Sherry,
    • 2 replies
    • 95 views

  11. Dark blue staining of ELIspot membrane

    By Guest Ellie,
    • 1 reply
    • 116 views

  12. Weird ELISPOT development

    By Guest Elias,
    • 3 replies
    • 208 views

  13. shelf life of pre-coated plates

    By Guest Stanislava,
    • 1 reply
    • 146 views

  14. Use of FCS for blocking

    By Guest thomson,
    • 3 replies
    • 124 views

  15. Spots in no stimuli wells when using mouse IFNg splenocytes

    By Guest Subeena Sood,
    • 4 replies
    • 392 views

  16. too many negative spot of IFNg ELISpot

    By mKim,
    • 2 replies
    • 228 views

  17. Colors of IFNg spot

    By Guest Tan Trieu Nguyen,
    • 1 reply
    • 234 views

  18. Using APCs in ELISpot with CD8+ T cells

    By Guest Nils,
    • 1 reply
    • 187 views

  19. Purple wells when developing Mouse IFN-y ELISPOT plus kit 3321-4AST-2

    By Guest Rongji,
    • 1 reply
    • 1.4k views

  20. IFNg ELISPOT: Inhibition of background spots in wells for which the donor is seronegative

    By Guest José Manuel Marín,
    • 1 reply
    • 242 views

  21. Poorly developed ELISpot membrane

    By Guest Lena,
    • 3 replies
    • 403 views

  22. Purple spot background

    By Guest Idania,
    • 1 reply
    • 351 views

  23. High Background in IFNg ELISpot for mouse spleenocytes

    By Guest Sajib,
    • 5 replies
    • 432 views

  24. Background Problem with Mouse IFN-gamma ELISPOT assay

    By Guest Pavel,
    • 9 replies
    • 2.6k views

  25. Antigen-specific IgG spots by antibody-negative donors

    By Keisuke,   
    • 4 replies
    • 261 views
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