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ELISpot

Questions and comments on ELISpot.

121 topics in this forum

  1. Result is negative

    By Sumu,
    • 0 replies
    • 5 views

  2. Treatment and long-term preservation of PVDF plates

    By Guest manny,
    • 4 replies
    • 36 views

  3. Problems with central membrane white spot and retained blue color

    By Guest Saswati Bhattacharya,
    • 3 replies
    • 443 views

  4. Cross-reactivity/ staining of membrane by secondary antibody

    By Guest Amalie,
    • 3 replies
    • 247 views

  5. Human pan IgG ELISpot - dark membrane and white spots

    By Guest Emily,
    • 2 replies
    • 147 views

  6. Validation of ELISpot

    By Guest Margot,
    • 0 replies
    • 74 views

  7. Effector target ratio for low antigen load

    By Gael,
    • 1 reply
    • 93 views

  8. Peptide length for ELISPOT?

    By Guest Guest Andrew,
    • 4 replies
    • 369 views

  9. IFN-gamma Elispot with human PBMC activated by adherent tumor cells

    By Guest Esen Aral,
    • 1 reply
    • 251 views

  10. Question about viability of frozen splenocytes after thawing (ELISpot)

    By Guest A Le Heron,
    • 1 reply
    • 134 views

  11. Odd spots in negative control wells

    By Guest Tim,
    • 1 reply
    • 117 views

  12. Help with antigen-specific ELISpot assay

    By Guest Federico Ruiz Moreno,
    • 6 replies
    • 395 views

  13. How long can a coated plate last in the fridge?

    By Guest Emily,
    • 2 replies
    • 269 views

  14. Question about RPMI and cell volume

    By Guest Federico Ruiz Moreno,
    • 1 reply
    • 244 views

  15. Issues with Elispot

    By Saswati Emma Mohr lab,
    • 1 reply
    • 359 views

  16. Buffer solution used for making coating anti human IgG for B cells ELISpot

    By Guest Syabila,
    • 3 replies
    • 413 views

  17. TMB for HRP SAME FOR ELISA AND ELISPOT

    By Guest Camila Enriquez,
    • 1 reply
    • 644 views

  18. Plate Preparation protocol of using Pre-coated ELISpot clear plate

    By Guest Syabila,
    • 5 replies
    • 303 views

  19. Blurry spots in antigen-specific wells

    By Guest Mason,
    • 2 replies
    • 304 views

  20. No clear spots in mouse IFN-γ ELISpot

    By Guest Rain,
    • 5 replies
    • 375 views

  21. IFN-g ELISpot using TMB

    By Guest Hayan,
    • 1 reply
    • 235 views

  22. MICE ELISpot Assay

    By Guest Ivan Ssali,
    • 1 reply
    • 273 views

  23. Shelf life of no pre-coated plates.

    By Guest Mathilde,
    • 2 replies
    • 417 views

  24. TMB used in B cell ELISpot development (3825-2H)

    By Guest Mason,
    • 5 replies
    • 527 views

  25. change of background color after development(B cell ELISpot)

    By Guest Eunju Jung,
    • 1 reply
    • 275 views
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