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ELISpot

Questions and comments on ELISpot.

122 topics in this forum

  1. Result is negative

    By Sumu,
    • 4 replies
    • 133 views

  2. 'Cells alone'/'no stimuli' wells with high number of spots

    By Guest Carlos,
    • 6 replies
    • 612 views

  3. Validation of ELISpot

    By Guest Margot,
    • 1 reply
    • 119 views

  4. preservation of pre-coated plates

    By Guest manny,
    • 1 reply
    • 61 views

  5. Treatment and long-term preservation of PVDF plates

    By Guest manny,
    • 5 replies
    • 143 views

  6. Problems with central membrane white spot and retained blue color

    By Guest Saswati Bhattacharya,
    • 3 replies
    • 485 views

  7. Cross-reactivity/ staining of membrane by secondary antibody

    By Guest Amalie,
    • 3 replies
    • 397 views

  8. Human pan IgG ELISpot - dark membrane and white spots

    By Guest Emily,
    • 2 replies
    • 172 views

  9. Effector target ratio for low antigen load

    By Gael,
    • 1 reply
    • 123 views

  10. Peptide length for ELISPOT?

    By Guest Guest Andrew,
    • 4 replies
    • 404 views

  11. IFN-gamma Elispot with human PBMC activated by adherent tumor cells

    By Guest Esen Aral,
    • 1 reply
    • 280 views

  12. Question about viability of frozen splenocytes after thawing (ELISpot)

    By Guest A Le Heron,
    • 1 reply
    • 166 views

  13. Odd spots in negative control wells

    By Guest Tim,
    • 1 reply
    • 140 views

  14. Help with antigen-specific ELISpot assay

    By Guest Federico Ruiz Moreno,
    • 6 replies
    • 433 views

  15. How long can a coated plate last in the fridge?

    By Guest Emily,
    • 2 replies
    • 310 views

  16. Question about RPMI and cell volume

    By Guest Federico Ruiz Moreno,
    • 1 reply
    • 266 views

  17. Issues with Elispot

    By Saswati Emma Mohr lab,
    • 1 reply
    • 391 views

  18. Buffer solution used for making coating anti human IgG for B cells ELISpot

    By Guest Syabila,
    • 3 replies
    • 423 views

  19. TMB for HRP SAME FOR ELISA AND ELISPOT

    By Guest Camila Enriquez,
    • 1 reply
    • 668 views

  20. Plate Preparation protocol of using Pre-coated ELISpot clear plate

    By Guest Syabila,
    • 5 replies
    • 313 views

  21. Blurry spots in antigen-specific wells

    By Guest Mason,
    • 2 replies
    • 338 views

  22. No clear spots in mouse IFN-γ ELISpot

    By Guest Rain,
    • 5 replies
    • 406 views

  23. IFN-g ELISpot using TMB

    By Guest Hayan,
    • 1 reply
    • 255 views

  24. MICE ELISpot Assay

    By Guest Ivan Ssali,
    • 1 reply
    • 295 views

  25. Shelf life of no pre-coated plates.

    By Guest Mathilde,
    • 2 replies
    • 452 views
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