Jump to content

ELISpot

Questions and comments on ELISpot.

126 topics in this forum

  1. Limit of detection for Human IFN-g/IL-4 FluoroSpot Flex Kit (Catalog X-01A16B-10)

    By Guest Maria,
    • 1 reply
    • 83 views

  2. Question: The spots in 3 wells are blue

    By mKim,
    • 1 reply
    • 101 views

  3. Wells appear “marbled” after developing

    By Guest Maxi,
    • 3 replies
    • 169 views

  4. Frozen, cryo-preserved cells

    By Guest TKN,
    • 1 reply
    • 121 views

  5. Result is negative

    By Sumu,
    • 4 replies
    • 215 views

  6. 'Cells alone'/'no stimuli' wells with high number of spots

    By Guest Carlos,
    • 6 replies
    • 724 views

  7. Validation of ELISpot

    By Guest Margot,
    • 1 reply
    • 171 views

  8. preservation of pre-coated plates

    By Guest manny,
    • 1 reply
    • 154 views

  9. Treatment and long-term preservation of PVDF plates

    By Guest manny,
    • 5 replies
    • 264 views

  10. Problems with central membrane white spot and retained blue color

    By Guest Saswati Bhattacharya,
    • 3 replies
    • 609 views

  11. Cross-reactivity/ staining of membrane by secondary antibody

    By Guest Amalie,
    • 3 replies
    • 509 views

  12. Human pan IgG ELISpot - dark membrane and white spots

    By Guest Emily,
    • 2 replies
    • 307 views

  13. Effector target ratio for low antigen load

    By Gael,
    • 1 reply
    • 156 views

  14. Peptide length for ELISPOT?

    By Guest Guest Andrew,
    • 4 replies
    • 517 views

  15. IFN-gamma Elispot with human PBMC activated by adherent tumor cells

    By Guest Esen Aral,
    • 1 reply
    • 423 views

  16. Question about viability of frozen splenocytes after thawing (ELISpot)

    By Guest A Le Heron,
    • 1 reply
    • 332 views

  17. Odd spots in negative control wells

    By Guest Tim,
    • 1 reply
    • 216 views

  18. Help with antigen-specific ELISpot assay

    By Guest Federico Ruiz Moreno,
    • 6 replies
    • 516 views

  19. How long can a coated plate last in the fridge?

    By Guest Emily,
    • 2 replies
    • 439 views

  20. Question about RPMI and cell volume

    By Guest Federico Ruiz Moreno,
    • 1 reply
    • 351 views

  21. Issues with Elispot

    By Saswati Emma Mohr lab,
    • 1 reply
    • 446 views

  22. Buffer solution used for making coating anti human IgG for B cells ELISpot

    By Guest Syabila,
    • 3 replies
    • 555 views

  23. TMB for HRP SAME FOR ELISA AND ELISPOT

    By Guest Camila Enriquez,
    • 1 reply
    • 785 views

  24. Plate Preparation protocol of using Pre-coated ELISpot clear plate

    By Guest Syabila,
    • 5 replies
    • 422 views

  25. Blurry spots in antigen-specific wells

    By Guest Mason,
    • 2 replies
    • 386 views
×
×
  • Create New...