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ELISpot

Questions and comments on ELISpot.

87 topics in this forum

  1. High background level and Repeatability

    By Xian Yang,
    • 3 replies
    • 24 views

  2. 'Cells alone'/'no stimuli' wells with high number of spots

    By Guest Carlos,
    • 4 replies
    • 74 views

  3. Rabbit IFN-r ELISPOT-Abnormal dark background

    By Guest Sherry,
    • 2 replies
    • 32 views

  4. Dark blue staining of ELIspot membrane

    By Guest Ellie,
    • 1 reply
    • 54 views

  5. Weird ELISPOT development

    By Guest Elias,
    • 3 replies
    • 133 views

  6. shelf life of pre-coated plates

    By Guest Stanislava,
    • 1 reply
    • 82 views

  7. Use of FCS for blocking

    By Guest thomson,
    • 3 replies
    • 54 views

  8. Spots in no stimuli wells when using mouse IFNg splenocytes

    By Guest Subeena Sood,
    • 4 replies
    • 248 views

  9. too many negative spot of IFNg ELISpot

    By mKim,
    • 2 replies
    • 148 views

  10. Colors of IFNg spot

    By Guest Tan Trieu Nguyen,
    • 1 reply
    • 173 views

  11. Using APCs in ELISpot with CD8+ T cells

    By Guest Nils,
    • 1 reply
    • 133 views

  12. Purple wells when developing Mouse IFN-y ELISPOT plus kit 3321-4AST-2

    By Guest Rongji,
    • 1 reply
    • 1.2k views

  13. IFNg ELISPOT: Inhibition of background spots in wells for which the donor is seronegative

    By Guest José Manuel Marín,
    • 1 reply
    • 197 views

  14. Poorly developed ELISpot membrane

    By Guest Lena,
    • 3 replies
    • 319 views

  15. Purple spot background

    By Guest Idania,
    • 1 reply
    • 272 views

  16. High Background in IFNg ELISpot for mouse spleenocytes

    By Guest Sajib,
    • 5 replies
    • 324 views

  17. Background Problem with Mouse IFN-gamma ELISPOT assay

    By Guest Pavel,
    • 9 replies
    • 2.4k views

  18. Antigen-specific IgG spots by antibody-negative donors

    By Keisuke,   
    • 4 replies
    • 195 views

  19. Stimuli for ELISPOT

    By Guest Alok Tembhurne,
    • 11 replies
    • 1.1k views

  20. IFN-Gamma ELISPOT No Development?

    By Guest Eva,
    • 6 replies
    • 909 views

  21. HBsAg-specific B cell ELISPOT assay

    By Guest E.J. Jung,
    • 8 replies
    • 351 views

  22. Dark "clumps" in peptide-stimulated samples

    By Guest Tetiana,
    • 1 reply
    • 344 views

  23. Total mouse IgG ELISPOT - not many spots?

    By Guest Lucy,
    • 9 replies
    • 300 views

  24. How long stable is the SFC on plate membrane after air-dry

    By mKim,
    • 2 replies
    • 206 views

  25. IQ/OQ of Eli Scan Plus reader

    By mKim,
    • 2 replies
    • 452 views
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