Effector target ratio for low antigen load

[Gael]

Hi,

I'm expecting low antigen load from my targets (cancer cell lines), what effector (T cells, good potency TCR) : Target ratio (cell number) and incubation time (24h-48h) do you recommend?

(1:1, 24h didn't work)

thank you,

Gaelle

 

[Christian@mabtech.com]

Hello Gael! 

People tend to use different ratio and it is best to set up a validation experiment where you titrate it out:

1:1
1:2

1:3

1:4

1:5

1:10

Also evaluate different cellnumbers. It might be needed to reach a certain cell-density before the killing can begin.