Guest Margot Posted March 15 Report Share Posted March 15 Hi, I have developed an ELISpot protocol that seems to be working quite well. Before I apply the protocol to test more of my precious patient samples, I wanted to check if there are (official) rules for the validation of ELISpot assays in terms of intra and inter-assay variability? I can see some variability between assays, but I suspect this is because my counting may not be that accurate. I now use tryptan blue staining and manual counting to determine viabilty and cells/mL which might not be ideal as it induces variability. What other methods are recommended? Quote Link to comment Share on other sites More sharing options...
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