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Plate Preparation protocol of using Pre-coated ELISpot clear plate

Guest Syabila

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Guest Syabila

Dear Mabtech representative,

I'd like to ask question about the protocol of using Pre-coated ELISpot clear plate (code: 3420-3PT) specifically a the plate preparation step. The Guidlines stated to remove the plate from the sealed package and wash 4 times with sterile PBS (200 uL/well). Since I don't have the sterile PBS, is it okay to use sterile RPMI instead of sterile PBS? And what could be the consequences of using the RPMI as the substitute? And if using RPMI is not a good alternative, do you have any recommend of solution that can be used to prepare the plate?
Looking forward to hearing from you soon. 
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