Guest Syabila Posted September 12, 2023 Report Share Posted September 12, 2023 I would like to perform B Cells ELISpot using your product (Anti-human IgG mAbs (MT91/145), unconjugated. Would it be possible for you to explain how to prepare the coating buffer for making the coating solution? Is it okay to use the carbonate-bicarbonate buffer? It will be very helpful for us to the prepare the reagents. Thank you very much Regards, Syabila Quote Link to comment Share on other sites More sharing options...
admin Posted September 12, 2023 Report Share Posted September 12, 2023 We recommend diluting the capture antibody in PBS. Concentration of the capture mab solution is generally 10-15ug/ml. Add 100ul into each well of etoh activated PVDF plates. Quote Link to comment Share on other sites More sharing options...
Guest Syabila Posted September 12, 2023 Report Share Posted September 12, 2023 Hi there Just to make sure, do you mean PBS 1x? And I am also curious about what is the reason for using the PBS instead of carbonate bicarbonate buffer? Since we aware that PBS and carbonate-bicarbonate buffer have a very different pH where carbonate-bicarbonate buffer is more alkaline than PBS. I also want to make sure, is the concentration of EtOH needed to activate the PVDF plate is following the protocol provided by the plate manufacture? Thank you Quote Link to comment Share on other sites More sharing options...
Christian@mabtech.com Posted October 4, 2023 Report Share Posted October 4, 2023 Yes I mean PBS 1x. Antibodies are happier in PBS. Yes, the etoh concentration needed for PVDF membrane activation is provided by Millipore. More and more people are switching to pre-coated ELISpot plates and this is my strong recommendation. Guarantees optimal results. ELISpot most often goes wrong due to incorrect coating effects. Quote Link to comment Share on other sites More sharing options...
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