Guest Frank_K Posted October 19, 2015 Report Share Posted October 19, 2015 Dear Mabtech, If possible, I would like to have your input on one of our upcoming experiments. As part of a larger project, we would like to analyze responses from mouse Th1 and Th2 cells by ELISpot. For Th2, we would like to analyze IL-4, IL-5 and possibly IL-13. Here we are especially interested in IL-4. It is my understanding that this cytokine is easier to analyze with ELISpot compared to ELISA, is this correct? For Th1 cytokines, we plan to analyze IFN-g. Are there any other Th1 cytokines that are suitable to analyze by ELISpot? Also, I have a more technical question. We have a bunch of ELISpot plates called MAIPS4510 in our lab. I could not find these among the plates you recommend in your plate coating guide, but are these ok to use? Thanks a million! Regards, Frank Link to comment Share on other sites More sharing options...
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