Questions from customers Posted February 2, 2016 Report Share Posted February 2, 2016 Dear Mabtech Customer Service Team! We are routinely using your Mabtech IFNg ELISpot kits (#3420-2APT-2 or #3420-2AST-2) for our experiments. Recently, we observed some strange looking spots we don`t have an explanation for, maybe you can provide me with your expert opinion on this? Experiment set-up: purified monocytes are matured to dendritic cells; these are then loaded with peptides and incubated together with purified CD8+ T cells on the IFNg ELISpot plates. Usually we get distinct spots; however, in the last experiment we observed some kind of clustering of cells (see the attached powerpoint), and we don`t know how to interpret these data. Maybe you can help us? Do you know how such clustered spots might be caused? Thank you very much and best regards, G Examples of ELISPOT trails.pptx Link to comment Share on other sites More sharing options...
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