Francesca Posted November 22, 2016 Report Share Posted November 22, 2016 Dear all I would like to perform an Elispot to measure the memory B cells in patients who received (1 year ago) the 13 valent pneumococcal vaccine (prevenar). I’ll use the kit from mabtech with code MBT-3850-2A (ALP) and I’ll coat the 96-plate (Millipore MSIPS4510 treated with EtOH) with the antigen , consisting in each serotype included in prevenar (pneumococcal polysaccharide serotype 1, 3, 4, 5, 6A , 6B , 7F, 14, 18, 19A, 19F, 23F). I’ll coat a plate with single 13 serotypes (in triplicate 13 X3 =39 wells), control negative and IgG (as a positive control). -I wonder if I need to conjugate each pneumococcal polysaccharide with human methylated albumin during the coating. -do I need to assay a negative control for each serotype? Can I use in the plate just one well with negative control coated with one serotype? Thanks a lot Francesca Link to comment Share on other sites More sharing options...
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