Guest Rafael Blasco Posted February 13, 2017 Report Share Posted February 13, 2017 I have been using your kit for human INF-gamma ELISPOT-ALPplus. My experimental settings require stimulating the PBMCs one week before performing the ELISPOT and I am using human AB serum during this experiment. My first three ELISPOT worked very well until I changed the serum. I have tried two stocks after that and both gave me high background, is there any way to solve this other than the obvious answer of trying more stocks of serum. Thank you very much Link to comment Share on other sites More sharing options...
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