Guest Janna Posted November 15, 2017 Report Share Posted November 15, 2017 Hi, I have been working with the mouse IgG elispot kit (product code 3825-H) to detect both total IgG and ag-specific IgG to a virus-like particle (VLP) vaccine candidate in mouse splenocytes. For the antigen-specific, I coat my plates with 5ug/ml of the VLP. In both protocols, I am seeing a very uneven background signal both within and between wells. This problem is more pronounced with the ag-specific assay, but is also present with the total IgG assay. I attached a picture of the dry plate. As you can see, in some wells there is a stripe of dark purple background, with no background in the rest of the well. In other wells, there is no background at all. Do you have any ideas why this might be happening? Thank you! Link to comment Share on other sites More sharing options...
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