Guest Eunju, Jung Posted December 6, 2017 Report Share Posted December 6, 2017 Dear, Mabtech team I am measuring influenza antigen-specific antibody secreting cell using Human IgG ELISpot. But I noticed that spots were not clear and some spots were shown as a clump. I am using cryopreserved PBMC and when thawing PBMC, I use DNase to prevent cell clumping. Is there anything I can do to improve blurred spots? Or will it go well, if I try increasing a concentrate of DNase? In attached ppt file, first slide is a result of spots development during 15 minutes. As 15 minutes, background was too darken to distinguish spots. So I shortened the development time. Next slide is results of two developments during 3min, 30sec and 1min,45sec. Please give me a help with this spot problem. Sincerely, Eunju, Jung B cell ELISPOT.pptx Link to comment Share on other sites More sharing options...
This topic is now archived and is closed to further replies.