Guest Eunju, Jung Posted December 15, 2017 Report Share Posted December 15, 2017 Hi, thank you for your kind answer about previous my question. I'm writing again to ask another question. It's first time to me conducting B cell ELISPOT, so many things are confusing me. I wounder the positive control (PC) count reference value. I will use the Human IgG ELISPOT basic (product code:3850-2H) to measure influenza Ag specific memory B cell and will use monoclonal antibody MT91/145 as a positive control. I'm using cryopreserved PBMC and blood samples were drawn at prevaccination and 28 days post vaccination against influenza. And I will plate cells at 10,000/100ul per well for positive control. I will compare cell response at prevaccination and 28 days post vaccination, and I think PC may be different with each other. I hope you can give an information about PC reference value. It will be a big help. Sincerely, Eunju, Jung Link to comment Share on other sites More sharing options...
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