Guest Rongji Posted November 16, 2021 Report Share Posted November 16, 2021 Hi, I was doing the last step for colour development when I saw many wells turning purple. Does it affect the development of the spots? Thank you. Rongji Quote Link to comment Share on other sites More sharing options...
Christian@mabtech.com Posted November 16, 2021 Report Share Posted November 16, 2021 A purple tint to the ELISpot membrane after substrate development is rather common. It does not impact performance of the assay. However, maybe you can upload an image so I can have a look? Quote Link to comment Share on other sites More sharing options...
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