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ELISpot

Questions and comments on ELISpot.

118 topics in this forum

  1. 'Cells alone'/'no stimuli' wells with high number of spots

    By Guest Carlos,
    • 4 replies
    • 517 views

  2. About precipitate, membrane staining and negative controls in ELISpot assay

    By Yaice Sandoval,
    • 5 replies
    • 1.5k views

  3. Antigen-specific B cell ELISPOT

    By akira,
    • 12 replies
    • 1.1k views

  4. Antigen-specific IgG spots by antibody-negative donors

    By Keisuke,   
    • 4 replies
    • 498 views

  5. B cell ELISpot on specific antigen pre-coated ELISA plates

    By Guest Tyler,
    • 7 replies
    • 1.9k views

  6. B cell ELISpot questions

    By sebbeols,   
    • 5 replies
    • 10.8k views

  7. B cell ELISpot- Positive Control

    By ShawnAbeynaike,   
    • 7 replies
    • 2.5k views

  8. B cell ELISPOT, blurry spot

    By Guest Eunju, Jung,
    • 1 reply
    • 1.6k views

  9. B cell ELISPOT, positive control

    By Guest Eunju, Jung,
    • 1 reply
    • 968 views

  10. B-cell ELISpot IgG dark background staining

    By Guest Michelle,
    • 1 reply
    • 810 views

  11. Background Problem with Mouse IFN-gamma ELISPOT assay

    By Guest Pavel,
    • 9 replies
    • 3.2k views

  12. Biotinylating Ag for B Cell ELISpot

    By sebbeols,
    • 4 replies
    • 3.1k views

  13. Blank areas in IFNg ELISpot with human PBMCs

    By Guest Lena,
    • 4 replies
    • 665 views

  14. Blank wells after development

    By Questions from customers,
    • 5 replies
    • 2.5k views

  15. Blurry spots in antigen-specific wells

    By Guest Mason,
    • 2 replies
    • 257 views

  16. Bovine ASC elispot

    By Guest Analía,
    • 8 replies
    • 397 views

  17. Buffer solution used for making coating anti human IgG for B cells ELISpot

    By Guest Syabila,
    • 3 replies
    • 354 views

  18. Can we used spot size or morphology to distinguish memory B cell from Plasma cells?

    By mazor,   
    • 7 replies
    • 5.2k views

  19. CD8 T cells & IFN gamma

    By Guest Mia,
    • 2 replies
    • 3.1k views

  20. change of background color after development(B cell ELISpot)

    By Guest Eunju Jung,
    • 1 reply
    • 248 views

  21. Clear vs. white ELISpot plates?

    By Matthew,
    • 2 replies
    • 6.2k views

  22. Colors of IFNg spot

    By Guest Tan Trieu Nguyen,
    • 1 reply
    • 421 views

  23. Dark "clumps" in peptide-stimulated samples

    By Guest Tetiana,
    • 1 reply
    • 715 views

  24. Dark blue staining of ELIspot membrane

    By Guest Ellie,
    • 1 reply
    • 378 views

  25. Dark membranes in IFN-g ELISpot

    By John_K,
    • 3 replies
    • 6.1k views
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